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Magnetic bead method Genomic DNA extraction Kit 50T

Magnatic bead method Swab Genomic DNA extraction Kit 50T reagent for PCR qPCR NGS

The Iclean DNA Extraction Kit provides rapid and efficient magnetic bead method to purify & extract DNA (including genomic, mitochondrial and viral DNA) from preserved tissues,saliva,body fluids,and buccal,cervical,skin cells,bacterial cell etc.Samples can be stored for up to 2 weeks at 4°C before processing without a noticeable loss in DNA yield or quality. High quality genomic DNA can be purified in 15 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 8 μgper buccal swab. Purified DNA, with approximately 20-30 kb, is suitable for downstream applications such as PCR or other enzymatic reactions.

Application:

For purification & isolation of DNA (including genomic, mitochondrial,bacterial,parasite & viral DNA) from tissues,saliva,body fluids,and buccal,cervical,skin cells,bacterial cell,tissues,swabs,CSF,body fluids,washed urine cells 

Note: The DNA yield varies and is dependent on several factors including the technique of the person taking the swab, whether the donor is a high or low shedder, and the type of swab used.

Kit Content, Storage and Stability:

Component Storage 50 preps
Buffer 1 RT 15 ml
Binding Buffer  RT 15 ml
Inhibitor Removing Buffer  RT 27 ml
Washing Buffer  RT 15 ml

Elution Buffer  RT 15 ml
Protease K (20mg/ml) -20oC 20mg (Dry powder)
Spead RT /
Collection Tube (2ml) RT 50

Specification of magnatic bead method Genomic DNA extraction Kit 50T

Specifications

Detail

Format

magnetic bead

DNA extraction

The binding capacity is more than 8 ug of Swab/saliva DNA

Processing time

Whole steps in 15 minutes. From Proteinase K treated sample to DNA extraction in 5 minutes or less

Required reagents and equipment

Microcentrifuge, Vortex, Proteinase K, Heatblock

High Purity gDNA

A260/A280 = 8μg

Sample sources

tissues,saliva,body fluids,and buccal,cervical,skin cells,bacterial cell,tissues,swabs,CSF,body fluids,washed urine cells

Downstream application

PCR, qPCR, Array, Southern Blots,other enzymatic reactions PCR, Restriction Endonuclease Digestions

Storage

dry at room temperature (15-25℃)


Buccal cells Extraction Ki-- Functional Test Data in our class 10,000 Lab:

Starting Material:

Human buccal swabs

Elution Volume:

500 µl

DNA Yield:

82 ng/µl in 500 µl (Normalized Buccal Cell Kit) or up to 8µg (Buccal Cell Kit)

The Lab report of DNA extraction volume, please feel free to contact us if you need.

Advantages:

1.magnetic bead-based technology to isolate genomic DNA without the need hazardous chemicals, centrifugation, or vacuum manifolds,phenol and ethanol precipitation.

2.Rapid and efficient purification of genomic DNA from human buccal swabs in less than 15 minutes following sample preparation and lysis

3.Simple lysis with Proteinase K without the need for any mechanical lysis

4.Minimal contamination with RNA

5.The purified genomic DNA demonstrates improved downstream performance in applications including PCR

6.Includes a kit designed for automated processing of large numbers of samples in 96-well plates using a liquid handling robot

Individual samples Processing

Materials requested:

-A magnetic separation rack suitable for use with 1.5 ml microcentrifuge tubes (see below)

-Sterile, 1.5 ml microcentrifuge tubes

-Vortex mixer

-20 µl, 200 µl, and 1 ml sterile, pipette tips

-Water bath at 37°C

Magnetic Rack
 
The Magnatic Rack is a two-piece magnetic separation rack for use in protocols with magnetic beads, and consists of a magnetic base station and a removable tube rack. The tube rack can hold 8 microcentrifuge tubes. The tube rack fits onto the magnetic base station in two different positions, associating the row of 12 neodymium magnets with a single row of 4 tubes for simple ‘on the magnet’ and ‘off the magnet’ sample processing. 

Automated Sample Processing:
Use DNA Extraction Kits process large numbers of samples in 96-well format using an automated liquid handling robot.avoiding the need for centrifugation steps or the use of ethanol or chaotropic salts.  

Hardware Requested:
-liquid handling robotic workstation with a gripper arm

-Appropriate tips for liquid dispensing and aspiration (see below for factors to consider)

-96-Well Magnetic Separator

-Shaker

-Incubator, heat block, or water bath for heating samples

-96 x 2 ml deep well plate(s) 

-96 x 300 µl U-Bottomed microtiter plate