HCY™ Canine Coronavirus (CCoV) Real-time PCR Detection Kit (Lyophilized Bead)

HCY™  Canine Coronavirus PCR Detection Kit

• Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
• The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
• Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
• Easy to use, no need to prepare reagents in advance;
• The minimum detection limit is 4 copies/Test;
• Clinical sensitivity 100%, specificity 100%;
• Add internal standard to prevent false negative results and facilitate indoor quality control.

Quality by Design

Canine Coronavirus (CCoV) has both intestinal and respiratory subtypes, CCOV and CRCV, and CCoV-Ⅰ and CCoV-Ⅱ, CCoV-Ⅱ is divided into two genotypes, CCoV-Ⅱa and CCoV-Ⅱb. We compared the 1508 CCOV sequences in NCBI GenBank, 440 sequences in China, and the reference sequence CD19_21010626 (OQ623693), and constructed an evolutionary tree for homology analysis. 21010626 (OQ623693) were compared, homology comparison was performed and evolutionary tree analysis was constructed.

HCY™ Canine coronavirus PCR Detection Kit is is designed and developed based on the above gene sequences of endemic pathogens in different regions of the world, which fully ensures the accuracy and validity of the test. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.

LoD and PCR Efficiency

Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 4 copies/Test. Calculation of the experimental data set yielded R²=0.999 and the amplification efficiency E=99.2%.

Sensitivity and Specificity

The HCY™ Canine coronavirus PCR Detection Kit was compared to similar commercially available products and tested 135 real clinical samples, of which the sensitivity was 100% and the specificity 100%.

		Reference Product		Total
		Negative	Positive
HCY™ PCR Test Kit	Positive	0	22	18
	Negative	117	0	117
Total		117	18	135

	Estimated Value	95% Confidence Interval
Sensitivity	100%	78.12%~100%
Specificity	100%	96.04%~100%

Cross-reactivity

The HCY™ Canine coronavirus PCR Detection Kit was validated against Bartonella, Borrelia Burgdorferi, Toxoplasmosis, Leptospira, Bordetella bronchiseptica, Feline Calicivirus, Feline coronavirus, Feline herpesvirus, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis, Chlamydia Felis, Parainfluenza Virus, Canine Herpesvirus, Canine Distempervirus, and Canine Parvovirus, all of which did not cross-react.