Test principle of the Canine Parvovirus Antigen Test Kit

Rapid immunochromatographic assay technique for the detection of canine cerebrospinal virus antigen. A rectal or fecal sample is obtained and added to the spiked wells and then moved along the chromatographic membrane with a colloidal gold-labeled anti-CPV monoclonal antibody. If the CPV antigen is present in the sample, it binds to the antibody on the test line and appears burgundy. If the CPV antigen is not present in the sample, no color reaction is produced.

The Canine Parvovirus Antigen Test Kit is able to detect Canine Parvovirus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Use

For rapid screening of Canine Parvovirus in the canine eye and conjunctival secretions, nasal fluid, saliva, feces, rectum, and vomit. (CPV).

Components of the FPV-Ag Test Kit

Item NO.	Test Cassette	Diluent	Disposable Swab	Instruction
CY-G008-1 (1 test)	1 pc	1 pc	1 pc	1 pc
CY-G008-10 (10 test)	10 pcs	10 pcs	10 pcs	1 pc
CY-G008-25 (25 tests)	25 pcs	25 pcs	25 pcs	1 pc

Storage conditions and Validity Period:

Store at 2-30°C, valid for 24 months.

Sample Requirements

• This kit is only suitable for testing canine fecal, rectal and vomit samples.
• The test sample is collected fresh and put into dilution solution immediately, and the diluted sample should be tested as soon as possible within 1 hour.
• If the sample cannot be tested in time, the diluted sample can be stored at 2-8℃ for 24 hours and -20℃ for 1 month.

How to Use Canine Parvovirus Antigen Test Kit

• Disposable swabs were used to collect samples of canine feces, rectum, and vomit. After collection, the samples were put into the dilution solution and stirred, left for 1 min, removed the swabs, tightened the lid, and mixed upside down.
• Tear open the aluminum foil bag of the test card, remove the test card and place it on a flat, clean counter top.
• Pipette 3 drops (about 80 μl) of the diluted sample into the spiked well of the test card and wait for the test result.
• Wait for 10-15 min at room temperature to read the test results, after 20 min the test results are invalid.

Interpretation of Results

Negative: Only the control line (C) has a purple-red band.

Positive: Both the test line (T) and the control line (C) appear purple-red bands.

Invalid: The control line (C) does not appear purple-red band.

NOTE: Invalid test results can be caused by a variety of reasons and should be re-tested.

Product Performance

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Precision: There are no more than one false negative and false positive judgment results within a batch; the color development of the test line and the control line between the three batch number test strips (cards) between batches should be uniform, and the color development between batches is not uniform. There is no more than one judgment result.

Stability: 2-30 ℃ unopened validity period of 2 years

Precautions for using the Canine Parvovirus Antigen Test Kit

1. Please read the instructions carefully before the test. The various reagents provided by this product are only for this test.
2. Products with expired or damaged aluminum foil bags cannot be used.
3. If the kit is taken out under low temperature storage, it needs to be returned to room temperature before opening, and the opened test card should be used as soon as possible to avoid moisture, and it should not exceed 1 hour.
4. Avoid touching the sample well on the test card and the chromatographic strip at the test window.
5. The waste after the test should be regarded as pollutants and properly disposed of according to relevant local regulations.

Test principle of Canine Parvovirus Antibody Test Kit

The kit is made by the principle of colloidal gold immunochromatography. After the sample is added to the sample hole, it moves along the chromatographic membrane together with the colloidal gold marker. If there is CPV antibody in the sample, the detection line will display purple-red. If there is no CPV in the sample Antibodies do not produce a color reaction.

The Canine Parvovirus test kit is able to detect Canine Parvovirus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only. And the quantitative results are trackable.

Intend Use

For rapid screening of Canine Parvovirus in canine whole blood, serum, or plasma (CPV).

Components of the CPV-Ab Test Kit

Item NO.	Test Cassette	Finger-stick Device	Assay Buffer	Finger-stick Device	Diluent	Instruction
CY-G007-1 (1 test)	1 pc	1 pc	1 pc	1 pc	1 pc	1 pc
CY-G007-10 (10 tests)	10 pcs	10 pcs	10 pcs	10 pcs	10 pcs	1 pc
CY-G007-25 (25 tests)	25 pcs	25 pcs	25 pcs	25 pcs	25 pcs	1 pc

Storage Conditions and Validity Period

Store at 2-30°C, valid for 24 months.

Sample Requirements

• This kit is only suitable for testing canine whole blood, serum or plasma samples.
• Test samples Collect fresh samples and should be tested as soon as possible within 1 hour.
• If not detected in time, whole blood, serum or plasma samples can be stored at 2-8°C for 3 days, and serum or plasma samples can be stored at -20°C for 1 month.

How to use the Canine Parvovirus Antibody Test Kit？

• Collect dog whole blood, serum or plasma samples, add the samples to the diluent after collection, tighten the lid, and mix by inversion.
• Tear open the aluminum foil bag of the test card, take out the test card, and place it on a flat and clean table.
• Pipette 3 drops (about 80 μl) of the diluted sample into the sample hole of the test card and wait for the test result.
• Wait 10-15 minutes at room temperature to interpret the test results, and the test results after more than 20 minutes are invalid.

Remarks: The above interpretation time is carried out at room temperature of 15-30°C. If the temperature is lower than 15°C, the interpretation time should be appropriately extended; if the temperature is higher than 30°C, the interpretation time should be appropriately shortened.

Interpretation of Results

Negative: Within the detection window, only the control line (C) has a purple-red band.

Positive: Within the detection window, both the test line (T) and the control line (C) appear purple-red bands.

Invalid: In the detection window, the control line (C) does not appear purple-red band.

• A negative test result simply means that there are no canine parvovirus antibodies in the test sample.
• The test result was positive surface test samples containing canine parvovirus antibodies.
• Invalid test results can be caused by a variety of reasons and should be re-tested.

Limitations of Detection Method

This kit can only detect whether there are canine parvovirus antibodies in the tested sample.
The test results of this kit are for clinical reference only and are not used as a basis for diagnosis.

Product Performance

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Precision: There are no more than one false negative and false positive judgment results within a batch; the color development of the test line and the control line between the three batch number test strips (cards) between batches should be uniform, and the color development between batches is not uniform. There is no more than one judgment result.

Stability: 2-30 ℃ unopened validity period of 2 years

Precautions for using the Canine Parvovirus Antibody Test Kit

1. Please read the instructions carefully before the test. The various reagents provided by this product are only for this test.
2. Products with expired or damaged aluminum foil bags cannot be used.
3. If the kit is taken out under low-temperature storage, it needs to be returned to room temperature before opening, and the opened test card should be used as soon as possible to avoid moisture, and it should not exceed 1 hour.
4. Avoid touching the sample well on the test card and the chromatographic strip at the test window.
5. The waste after the test should be regarded as pollutants and properly disposed of according to relevant local regulations.