1. What is a blood collection card?

A blood collection card (also known as a dried blood spot collection card, Dried Blood Spot, DBS) is a paper or cellulose-based card specially used for collecting, storing and transporting trace blood samples. It forms a dried blood spot by absorbing a small amount of blood (usually 50-100μL) and drying it naturally in the air, which is convenient for long-term storage and subsequent laboratory testing.

Main components
Filter paper substrate: high adsorption, no heavy metal pollution
Marking area: clear blood collection location (circle with a diameter of about 1cm)
Anti-fouling layer: prevent cross-contamination
Tearable design: convenient for laboratory sampling
II. Working principle of blood collection card

1. Blood adsorption and drying
   Collect blood by fingertip or heel puncture
   Blood drops on the marked area of ​​the card, naturally penetrate and spread
   Dry at room temperature for 2-4 hours (avoid direct sunlight)
2. Blood spot stabilization
   Hemoglobin, DNA, protein and other components are fixed in the fiber matrix
   Some collection cards contain chemical stabilizers (such as EDTA) to prevent degradation
3. Laboratory testing process
   Punching sampling: use a punch to take a blood spot of fixed diameter (3-6mm)
   Elution extraction: dissolve the target (such as DNA, antibodies, metabolites) with buffer
   Analysis and detection: PCR, ELISA, mass spectrometry, etc.
   III. Core advantages of blood collection card
   ✔ Micro-sampling: only 50-100μL (traditional blood collection tubes require 1-5mL)
   ✔ Strong stability: can be stored at room temperature for weeks to months (some tests up to 1 year)
   ✔ Convenient transportation: no cold chain required, reducing logistics costs
   ✔ Wide applicability: can be used for DNA, RNA, antibodies, metabolite detection
   ✔ Non-invasive/minimally invasive: especially suitable for newborns and field surveys

IV. Application areas of blood collection cards

1. Neonatal screening
   Phenylketonuria (PKU)

Congenital hypothyroidism

Genetic metabolic disease detection

2. Disease monitoring and epidemiological research
   HIV, hepatitis B, malaria antibody detection

New coronavirus serological survey

3. Genetic and molecular testing
   Parenthood testing

Pharmacogenomic analysis

4. Drug monitoring
   Detection of therapeutic drug concentration (such as anti-epileptic drugs)

Drug abuse screening

V. Steps for correct use of blood collection cards
Preparation before blood collection

Disinfect the blood collection site (fingertips/heels) with alcohol

Use a disposable blood collection needle

Blood dripping operation

Let the blood flow out naturally and touch the card marking area

Avoid repeated application to prevent hemolysis

Drying and storage

Place horizontally and air dry naturally (2-4 hours)

Put in a moisture-proof bag and add a desiccant

Transportation requirements

Avoid high temperature and humidity

It is recommended to use a sealed bag + shockproof packaging for long-distance transportation

VI. Common problems and solutions
❌ Problem 1: Uneven spread of blood spots
✅ Solution: Control the blood volume (do not exceed the circle range) and avoid repeated dripping

❌ Problem 2: Low detection sensitivity
✅ Solution: Ensure sufficient drying and avoid high temperature and high humidity environment

❌ Problem 3: Sample mildew
✅ Solution: Add mildew inhibitor or store at low temperature as soon as possible

VII. Product features of Huachenyang blood collection card
✔ Medical grade filter paper: meets WHO standards
✔ Pre-packaged desiccant: ready to use
✔ Multiple specifications:

Standard type (5-6 blood spots/card)

Newborn card (optimized design for heel blood sampling)

Anti-pollution type (with antibacterial coating)

DNA Testing

DNA testing refers to the deciphering of the genetic code carried by the human body through molecular biology and is mainly used to predict the probability of an individual suffering from a certain disease in the future, usually by taking a drop of blood, oral mucosa or other tissue cells from the person being tested.

In addition to regular medical checkups, cancer screening is also included in people’s daily medical checkup lists. If there is a tool or instrument that allows us to do the sampling at home, wouldn’t it be much more convenient? The buccal swab collection kit allows you to collect DNA at home.

Components of Buccal Swab Collection Kit

• Buccal swab (nylon flocked swab): 2pcs
• Sample preservation solution: 2pcs
• Barcode sticker: 4pcs
• Return bag: 1pc

How to use Buccal Swab Collection Kit

1. Check that the package of the buccal swab is undamaged and remove the buccal swab, taking care not to let the DNA buccal swab come into contact with other items to avoid contamination.

2. Wipe the buccal area of the mouth with a cheek swab about 20 times to ensure that enough oral mucosa is collected.

3. Place the nylon flocked swab tip in the sample preservation solution and break the swab handle at the break point.

4. Do it twice to get two tubes with DNA sample.

5. Put a barcode on the return bag.

6. Fill in the information.

7. Put the tubes in return bag and seal it.

8. Send DNA samples to the laboratory.

9. Get a lab report.

The specific items you want to test need to be decided according to your personal needs. The genetic sampling kit is used for genetic sampling before the test, so you can complete the collection of genes independently. DNA testing can help us prevent disease and give us a longer, healthier life.

Who is suitable for genetic testing?

• Those who have family history of disease and want to prevent it
• Those who have bad living habits and worry about cancer
• Those who have been exposed to chemical pollution, heavy metal pollution, nuclear pollution and exposure to toxic and harmful substances for a long time
• People who are concerned about physical health.
• People who are concerned about the growth of their children
• People who are concerned about intellectual development

What is the purpose of DNA testing?

The purpose of genetic testing is to “know early and prevent early” so that diseases that are likely to occur, especially hereditary diseases, do not appear. For example, the incidence of malignant tumors is gradually increasing, but the treatment effect is poor. After identifying genes that predispose to diseases such as malignant tumors, a scientific health assessment can be conducted and the best health care methods and lifestyle can be developed to avoid and prevent the occurrence of these major diseases as much as possible.

How to use the DNA Sampling Kit

Record the name of the subject and the test to see if they have heavy mouth ulcers or bronchial disease. If the subject has these conditions, taking a mouth swab for DNA collection is not recommended.

The subject is required to rinse his or her mouth with water three times and wait patiently for 30 minutes after the mouth is cleaned, without eating, drinking, smoking, or chewing gum.

After 30 minutes, check that the collection kit packaging is not broken and replace it if there is any damage.

The sampling kit will be given to the subject with the introduction of the genetic test, product classification instructions, specimen collection instructions, and other information (for minors, please leave it to parents to manage).

Attach barcodes to the genetic test save the card, information collection form, specimen tubes and return boxes, and give the save card with the barcode to the test subject for future report inquiries.

Have the subject or parent confirm that the personal information is correct and sign.