Test principle of the Canine Influenza Virus Antigen Test Kit

This Canine Influenza Virus Antigen Test Kit detects canine influenza virus antigen using a rapid immunochromatographic technique. After samples obtained from eye secretions, nasal fluids, and saliva are added to the test tape wells, the samples are moved along the chromatographic membrane together with a colloidal gold-labeled anti-CIV monoclonal antibody. The T detection line shows red if the CIV antigen is present in the sample. If the CIV antigen is not present in the sample, the T detection line does not appear in color.

The Canine Influenza Virus Antigen Test Kit is able to detect Canine Influenza Virus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Use

For rapid screening of Canine Influenza Virus in canine eye and conjunctival secretions, nasal fluid or saliva (CIV).

Components of the CIV-Ag Test Kit

Item NO.	Test Cassette	Diluent	Disposable Swab	Instruction
CY-G0012-1 (1 test)	1 pc	1 pc	1 pc	1 pc
CY-G0012-10 (10 tests)	10 pcs	10 pcs	10 pcs	1 pc
CY-G0012-25 (25 tests)	25 pcs	25 pcs	25 pcs	1 pc

Storage conditions and Validity Period:

Store at 2-30°C, valid for 24 months.

Sample Requirements

• This Canine Influenza Virus Antigen Test Kit is only suitable for testing eye secretions, nasal fluids, and saliva samples from dogs.
• The sample for the test must be collected immediately, the sample should be put into the dilution solution immediately after sampling, and the diluted sample should be tested as soon as possible, within 1 hour.
• Diluted samples can be stored at 2-8°C for 24 hours and can be stored at -20°C for one month.

How to Use Canine Influenza Virus Antigen Test Kit

1. Disposable swabs were used to collect samples of eye secretions, nasal fluids, and saliva from the dogs, and then the swabs were placed in the diluted solution and mixed and left to stand for 1 minute. Remove the swab, tighten the cap, and turn the sample tube upside down to mix.
2. Place the test strip on a flat, clean table.
3. Pipette 3 drops (approximately 80 μl) of sample onto the assay tape and wait for the test results.
4. Wait for 10-15 min at room temperature to read the test results. After 20 min, the test results are invalid.

Note: The above interpretation time is conducted at room temperature 15-30℃. If the temperature is lower than 15℃, the interpretation time should be delayed appropriately. If the temperature exceeds 30 ℃, the results should be read in advance.

Interpretation of Results

Negative: Only the C line appears as a red color.
Positive: Both T and C lines appear purplish red.
Invalid: No color appears in the C line.

NOTE: Invalid test results can be caused by a variety of reasons and should be re-tested.

Product Performance

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Stability: 2-30 ℃ unopened validity period of 2 years

Precautions for using the Canine Influenza Virus Antigen Test Kit

• The various reagents provided in this Canine Influenza Virus Antigen Test Kit are for this test only. Please read the instructions carefully before giving the test to your dog.
• Do not use it if the product has expired or the foil bag is damaged.
• If the Canine Influenza Virus Antigen Test Kit is removed from cryopreservation, it should be opened after returning to room temperature and used within 1 hour.
• Avoid contact with the sample wells and laminar strips on the test tape.

Test principle of the Canine Adenovims TypeⅠ Antigen Test Kit

This product uses rapid immunochromatography technology to detect canine adenovirus type I antigen in the secretions of the eyes, nose and mouth of dogs. If there was CAV I antigen in the sample, it would specifically bind to the recombinant protein labeled with colloidal gold to form a complex that moved along the chromatographic membrane and was captured by CAV I antibody coated on the membrane, showing a wine red line on the detection line (T). If the CAV I antigen is not present in the sample, no visible lines are formed at the T position. No matter the result is negative or positive, the C line should be colored, otherwise the result is invalid.

Components of the Infectious CAVⅠ-Ag Test Kit

Component	CY-CAVⅠ AG-01 (1 test)	CY-CAVⅠ AG-10 (10 tests)	CY-CAVⅠ AG-25 (25 tests)
Test device in foil bag (including test card,pipette &desiccant)	1 pc	10 pcs	25 pcs
Extraction vial/cap (contains extraction buffer solution)	1 vial and cap	10 vials and caps	25 vials and caps
Sampling swab	1 pc	10 pcs	25 pcs
IFU	1 pc	1 pc	1 pc

Storage conditions and Validity Period:

Store at 2-30°C and be valid for 24 months.

Sample Requirements of Canine Adenovims TypeⅠ Antigen Test Kit

• Please refer to the instructions for use
• This kit is only suitable for testing feline whole blood or serum samples (hemolysis and hyperlipidemia samples cannot be used).
• Collect fresh samples and immediately put into the extraction buffer solution, which should be detected as soon as possible within 1 hour.
• If samples were not timely detected, store within 24 hours at 2-8°C and store within 1 month at -20°C.

Product Performance Indifelineors

1. Sensitivity ≥ 95%.
2. Specificity ≥ 97.5%.
3. Precision: no more than one false negative and false positive results within the batch; the test lines & control line (cards) between three lots shall be uniform, and no more than one time of color difference between lots.
4. Stability: valid for 24 month if unopened & store 2-30°C.

Interpretation of Results

Negative: In the test window, only one fuchsia-colored line appear next to “C”.
Positive: In the test window, two distinct colored lines appear next to “T” & “C” simultaneously.
Invalid: No color line visible next to “C”.

NOTE: Invalid test results can be caused by a variety of reasons and should be re-tested.

Test principle of the Canine Adenovims TypeⅡ Antigen Test Kit

This product uses rapid immunochromatography technology to detect canine adenovirus typeⅡ antigen in the secretions from the eyes, nose and oral of dogs. After the diluted sample was dropped into the sample well, if there was CAVⅡ antigen in the sample, it would specifically bind to the recombinant protein labeled with colloidal gold to form a complex that moved along the chromatographic membrane and was captured by the CAVⅡ antibody coated on the membrane, showing a wine red line on the detection line (T). “If there is no CAVⅡ antigen in the sample, no visible line will be formed at the T position.” No matter the result is negative or positive, the C line should be colored, otherwise the result is invalid.

Components of the Infectious CAVⅡ-Ag Test Kit

Component	CY-CAVⅡAG-01 (1 test)	CY-CAVⅡAG-10 (10 tests)	CY-CAVⅡAG-25 (25 tests)
Test device in foil bag (including test card,pipette &desiccant)	1 pc	10 pcs	25 pcs
Extraction vial/cap (contains extraction buffer solution)	1 vial and cap	10 vials and caps	25 vials and caps
Sampling swab	1 pc	10 pcs	25 pcs
IFU	1 pc	1 pc	1 pc

Storage conditions and Validity Period:

Store at 2-30°C and be valid for 24 months.

Sample Requirements of Canine Adenovims TypeⅡ Antigen Test Kit

• Please refer to the instructions for use
• This kit is only suitable for testing feline whole blood or serum samples (hemolysis and hyperlipidemia samples cannot be used).
• Collect fresh samples and immediately put into the extraction buffer solution, which should be detected as soon as possible within 1 hour.
• If samples were not timely detected, store within 24 hours at 2-8°C and store within 1 month at -20°C.

Product Performance Indifelineors

1. Sensitivity ≥ 95%.
2. Specificity ≥ 97.5%.
3. Precision: no more than one false negative and false positive results within the batch; the test lines & control line (cards) between three lots shall be uniform, and no more than one time of color difference between lots.
4. Stability: valid for 24 month if unopened & store 2-30°C.

Interpretation of Results

Negative: In the test window, only one fuchsia-colored line appear next to “C”.
Positive: In the test window, two distinct colored lines appear next to “T” & “C” simultaneously.
Invalid: No color line visible next to “C”.

NOTE: Invalid test results can be caused by a variety of reasons and should be re-tested.

Test principle of the Canine Coronavirus Antigen Test Kit

The Canine Coronavirus Antigen Test Kit uses a rapid immunochromatographic assay to detect canine coronavirus antigen.
A rectal or fecal sample is added to the spiked wells and then moved along the chromatographic membrane with a colloidal gold-labeled anti-CCV monoclonal antibody. If the CCV antigen is present in the sample, it binds to the antibody on the test line and appears red. No color reaction is produced if the CCV antigen is not present in the sample.

The Canine Coronavirus Antigen Test Kit is able to detect canine coronavirus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Usec

For rapid screening of Canine Coronavirus in the feces and rectum (CCV).

Components of the CCV-Ag Test Kit

Item NO.	Test Cassette	Diluent	Disposable Swab	Instruction
CY-G013-1 (1 test)	1 pc	1 pc	1 pc	1 pc
CY-G013-10 (10 test)	10 pcs	10 pcs	10 pcs	1 pc
CY-G013-25 (25 tests)	25 pcs	25 pcs	25 pcs	1 pc

Storage conditions and Validity Period:

Store at 2-30°C, valid for 24 months.

Sample Requirements

• This kit is only suitable for the detection of ocular secretions, nasal fluids, and saliva samples.
• Test samples Collect fresh samples and put them in the diluent immediately. The diluted samples should be tested as soon as possible within 1 hour.
• If it cannot be detected in time, the diluted samples can be stored at 2-8°C for 24 hours and at -20°C for 1 month.

Detection Method

1. The canine coronavirus antigen Test Kit is only suitable for testing canine fecal and rectal samples.
2. The sample to be tested should be fresh and immediately put into the dilution solution. It should be noted that the diluted sample should be tested as much as possible within 1 hour.
3. If the sample cannot be tested in time, the diluted sample can be stored at 2-8 °C for 24 hours and at -20 °C for one month.

Interpretation of Results

Negative: Within the detection window, only a red band appears in the control line (C).
Positive: Within the detection window, red bands simultaneously appear on the test line (T) and the control line (C).
Invalid: In the detection window, no red band appears on the control line (C).

• A negative test result indicates no canine coronavirus antigen in the test sample, and canine coronavirus infection cannot be ruled out if related acute symptoms appear.
• The test result was a positive surface test sample containing canine coronavirus antigen.
• Since canine coronavirus vaccines are usually attenuated, the virus may temporarily increase in the body during the immunization process and may show a positive reaction, disappearing 3-10 days after vaccination. Dogs treated with monoclonal antibodies may have false positives for a long time due to the production of secondary antibodies against monoclonal antibodies in the body.
• Invalid test results may be caused by various reasons and should be re-tested.

Performance of Canine Coronavirus Antigen Test Kit

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Precision:

There should be no more than one false negative and false positive determination result within a batch; the color development of the test line and the control line between the three batch number test strips (cards) between batches should be uniform and consistent. There should be at most one judgment result for color inhomogeneity between batches.

Stability: 2-30 ℃ unopened validity period of 2 years

Limitations of Detection Method

This kit can only detect whether there is canine distemper virus antigen in the tested sample.

The test results of this kit are for clinical reference only and are not used as a basis for diagnosis.

Precautions for using the Canine Coronavirus Antigen Test Kit

1. Please read the instructions carefully before the test. The various reagents provided by this product are only for this test.
2. Products that have expired or have damaged aluminum foil bags cannot be used.
3. If the kit is taken out under low-temperature storage, it needs to be opened after returning to room temperature, and the opened test card should be used as soon as possible to avoid moisture and should not exceed 1 hour.
4. Avoid touching the chromatography test strips at the sample wells and detection windows on the test card.
5. The waste after the test should be regarded as a pollutant and properly disposed of by relevant local regulations.

Test principle of the Rabies Virus Antigen Test Kit

This product uses rapid immunochromatography technology to detect rabies virus antigen in the saliva of canine and feline. If the RBV antigen was present in the sample, it would specifically bind to the recombinant protein labeled with colloidal gold to form a complex that moved along the chromatographic membrane and was captured by the RBV antibody coated on the membrane, showing a wine red line on the detection line (T). If the RBV antigen is not present in the sample, no visible lines are formed at the T position. No matter the result is negative or positive, the C line should be colored, otherwise the result is invalid.

Components of the Infectious RBV-Ag Test Kit

Component	CY-RABAG-01 (1 test)	CY-RABAG-10 (10 tests)	CY-RABAG-25 (25 tests)
Test device in foil bag (including test card,pipette &desiccant)	1 pc	10 pcs	25 pcs
Extraction vial/cap (contains extraction buffer solution)	1 vial and cap	10 vials and caps	25 vials and caps
Sampling swab	1 pc	10 pcs	25 pcs
IFU	1 pc	1 pc	1 pc

Storage conditions and Validity Period:

Store at 2-30°C and be valid for 24 months.

Sample Requirements of Rabies Virus Antigen Test Kit

• Please refer to the instructions for use
• This kit is only suitable for testing feline whole blood or serum samples (hemolysis and hyperlipidemia samples cannot be used).
• Collect fresh samples and immediately put into the extraction buffer solution, which should be detected as soon as possible within 1 hour.
• If samples were not timely detected, store within 24 hours at 2-8°C and store within 1 month at -20°C.

Product Performance Indifelineors

1. Sensitivity ≥ 95%.
2. Specificity ≥ 97.5%.
3. Precision: no more than one false negative and false positive results within the batch; the test lines & control line (cards) between three lots shall be uniform, and no more than one time of color difference between lots.
4. Stability: valid for 24 month if unopened & store 2-30°C.

Interpretation of Results

Negative: In the test window, only one fuchsia-colored line appear next to “C”.
Positive: In the test window, two distinct colored lines appear next to “T” & “C” simultaneously.
Invalid: No color line visible next to “C”.

NOTE: Invalid test results can be caused by a variety of reasons and should be re-tested.

Principle of Canine Distemper Virus Antibody Test Kit

Canine Distemper Virus Antibody Test Kit is made by the principle of colloidal gold immunochromatographic test. After the sample is added to the test well, it moves along the chromatographic membrane together with the colloidal gold marker, and the detection line will appear red if CDV antibody is present in the sample, and no color will appear if CDV antibody is not present in the sample.

The Canine Distemper test kit is able to detect Canine Distemper Virus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Use

For rapid screening of canine distemper virus in canine whole blood, serum, or plasma (CDV).

Components of the CDV-Ab Test Kit

Item NO.	Test Cassette	Finger-stick Device	Assay Buffer	Finger-stick Device	Diluent	Instruction
CY-G009-1 (1 test)	1 pc	1 pc	1 pc	1 pc	1 pc	1 pc
CY-G009-10 (10 tests)	10 pcs	10 pcs	10 pcs	10 pcs	10 pcs	1 pc
CY-G009-25 (25 tests)	25 pcs	25 pcs	25 pcs	25 pcs	25 pcs	1 pc

Storage Conditions and Validity Period

Store at 2-30°C, valid for 24 months.

Sample Requirements

• This kit is only suitable for testing canine whole blood, serum or plasma samples.
• Test samples Collect fresh samples and should be tested as soon as possible within 1 hour.
• If not detected in time, whole blood, serum or plasma samples can be stored at 2-8°C for 3 days, and serum or plasma samples can be stored at -20°C for 1 month.

How to use Canine Distemper Virus Antibody Test Kit

• Collect dog whole blood, serum or plasma samples, add the samples to the diluent after collection, tighten the lid, and mix by inversion.
• Tear open the aluminum foil bag of the test card, take out the test card, and place it on a flat and clean table.
• Pipette 3 drops (about 80 μl) of the diluted sample into the sample hole of the test card and wait for the test result.
• Wait 10-15 minutes at room temperature to interpret the test results, and the test results after more than 20 minutes are invalid.

Remarks: The above interpretation time is carried out at room temperature of 15-30°C. If the temperature is lower than 15°C, the interpretation time should be appropriately extended; if the temperature is higher than 30°C, the interpretation time should be appropriately shortened.

Interpretation of Results

Negative: Within the detection window, only the control line (C) has a purple-red band.

Positive: Within the detection window, both the test line (T) and the control line (C) appear purple-red bands.

Invalid: In the detection window, the control line (C) does not appear purple-red band.

• A negative test result simply means that there are no canine parvovirus antibodies in the test sample.
• The test result was positive surface test samples containing canine parvovirus antibodies.
• Invalid test results can be caused by a variety of reasons and should be re-tested.

Limitations of Detection Method

This kit can only detect whether there are Canine Distemper Virus antibodies in the tested sample.
The test results of this kit are for clinical reference only and are not used as a basis for diagnosis.

Product Performance

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Precision: There are no more than one false negative and false positive judgment results within a batch; the color development of the test line and the control line between the three batch number test strips (cards) between batches should be uniform, and the color development between batches is not uniform. There is no more than one judgment result.

Stability: 2-30 ℃ unopened validity period of 2 years

Precautions for using the Canine Parvovirus Antibody Test Kit

• Please read the instructions carefully before the test. The various reagents provided by this product are only for this test.
• Products with expired or damaged aluminum foil bags cannot be used.
• If the kit is taken out under low-temperature storage, it needs to be returned to room temperature before opening, and the opened test card should be used as soon as possible to avoid moisture, and it should not exceed 1 hour.
• Avoid touching the sample well on the test card and the chromatographic strip at the test window.
• The waste after the test should be regarded as pollutants and properly disposed of according to relevant local regulations.

Test principle of the Canine Distemper Virus Antigen Test Kit

Rapid immunochromatographic detection technology for the detection of canine distemper virus antigens. After adding the samples of eye secretions, nasal fluid and saliva to the sample wells, they move along the chromatographic membrane together with the colloidal gold-labeled anti-CDV monoclonal antibody. If the CDV antigen is present in the sample, it will bind to the antibody on the detection line (T) and display a wine red color. If no CDV antigen is present in the sample, no color reaction occurs.

The Canine Distemper test kit is able to detect Canine Distemper Virus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Use

For rapid screening of canine distemper virus in the canine eye and conjunctival secretions, nasal fluid, or saliva (CDV).

Components of the CDV-Ag Test Kit

Item NO.	Test Cassette	Diluent	Disposable Swab	Instruction
CY-G010-1 (1 test)	1 pc	1 pc	1 pc	1 pc
CY-G010-10 (10 test)	10 pcs	10 pcs	10 pcs	1 pc
CY-G010-25 (25 tests)	25 pcs	25 pcs	25 pcs	1 pc

Storage conditions and Validity Period

Store at 2-30°C, valid for 24 months.

Sample Requirements

• This kit is only suitable for the detection of ocular secretions, nasal fluids, and saliva samples.
• Test samples Collect fresh samples and put them in the diluent immediately. The diluted samples should be tested as soon as possible within 1 hour.
• If it cannot be detected in time, the diluted samples can be stored at 2-8°C for 24 hours and at -20°C for 1 month.

Detection Method

1. Disposable cotton swabs were used to collect eye secretions, nasal fluids, and saliva samples. After collection, the samples were placed in the diluent and stirred for 1 min. The cotton swabs were taken out, the lids were tightened, and the samples were inverted and mixed.
2. Tear open the aluminum foil bag of the test card, take out the test card, and place it on a flat and clean table.
3. Pipette 3 drops (about 80 μl) of the diluted sample into the sample hole of the test card and wait for the test result.
4. Wait 10-15 minutes at room temperature to interpret the test results, and the test results after more than 20 minutes are invalid.
5. Remarks: The above interpretation time is carried out at room temperature of 15-30°C. If the temperature is lower than 15°C, the interpretation time should be appropriately extended; if the temperature is higher than 30°C, the interpretation time should be appropriately shortened.

Interpretation of Results

Negative: Within the detection window, only the control line (C) has a purple-red band.

Positive: Within the detection window, both the test line (T) and the control line (C) appear purple-red bands.

Invalid: In the detection window, the control line (C) does not appear purple-red band.

• A negative test result indicates that there is no canine distemper virus antigen in the test sample. If corresponding acute symptoms occur, canine distemper virus infection cannot be ruled out.
• The test result is positive surface test sample contains canine distemper virus antigen.
• Since canine distemper vaccines are usually attenuated vaccines, the virus may temporarily increase in the body during the immunization process and may show a positive reaction, which disappears 3-10 days after vaccination. Dogs treated with monoclonal antibodies may present false positives for a long time due to the production of secondary antibodies against monoclonal antibodies in their bodies.
• Invalid test results can be caused by a variety of reasons and should be re-tested.

Limitations of Detection Method

This kit can only detect whether there is canine distemper virus antigen in the tested sample.

The test results of this kit are for clinical reference only and are not used as a basis for diagnosis.

Product Performance

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Precision: There are no more than one false negative and false positive judgment results within a batch; the color development of the test line and the control line between the three batch number test strips (cards) between batches should be uniform, and the color development between batches is not uniform. There is no more than one judgment result.

Stability: 2-30 ℃ unopened validity period of 2 years

Precautions for using the Canine Distemper Virus Antigen Test Kit

1. Please read the instructions carefully before the test. The various reagents provided by this product are only for this test.
2. Products with expired or damaged aluminum foil bags cannot be used.
3. If the kit is taken out under low temperature storage, it needs to be returned to room temperature before opening, and the opened test card should be used as soon as possible to avoid moisture, and it should not exceed 1 hour.
4. Avoid touching the sample well on the test card and the chromatographic strip at the test window.
5. The waste after the test should be regarded as pollutants and properly disposed of according to relevant local regulations.

Test principle of the Canine Parvovirus Antigen Test Kit

Rapid immunochromatographic assay technique for the detection of canine cerebrospinal virus antigen. A rectal or fecal sample is obtained and added to the spiked wells and then moved along the chromatographic membrane with a colloidal gold-labeled anti-CPV monoclonal antibody. If the CPV antigen is present in the sample, it binds to the antibody on the test line and appears burgundy. If the CPV antigen is not present in the sample, no color reaction is produced.

The Canine Parvovirus Antigen Test Kit is able to detect Canine Parvovirus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Use

For rapid screening of Canine Parvovirus in the canine eye and conjunctival secretions, nasal fluid, saliva, feces, rectum, and vomit. (CPV).

Components of the FPV-Ag Test Kit

Item NO.	Test Cassette	Diluent	Disposable Swab	Instruction
CY-G008-1 (1 test)	1 pc	1 pc	1 pc	1 pc
CY-G008-10 (10 test)	10 pcs	10 pcs	10 pcs	1 pc
CY-G008-25 (25 tests)	25 pcs	25 pcs	25 pcs	1 pc

Storage conditions and Validity Period:

Store at 2-30°C, valid for 24 months.

Sample Requirements

• This kit is only suitable for testing canine fecal, rectal and vomit samples.
• The test sample is collected fresh and put into dilution solution immediately, and the diluted sample should be tested as soon as possible within 1 hour.
• If the sample cannot be tested in time, the diluted sample can be stored at 2-8℃ for 24 hours and -20℃ for 1 month.

How to Use Canine Parvovirus Antigen Test Kit

• Disposable swabs were used to collect samples of canine feces, rectum, and vomit. After collection, the samples were put into the dilution solution and stirred, left for 1 min, removed the swabs, tightened the lid, and mixed upside down.
• Tear open the aluminum foil bag of the test card, remove the test card and place it on a flat, clean counter top.
• Pipette 3 drops (about 80 μl) of the diluted sample into the spiked well of the test card and wait for the test result.
• Wait for 10-15 min at room temperature to read the test results, after 20 min the test results are invalid.

Interpretation of Results

Negative: Only the control line (C) has a purple-red band.

Positive: Both the test line (T) and the control line (C) appear purple-red bands.

Invalid: The control line (C) does not appear purple-red band.

NOTE: Invalid test results can be caused by a variety of reasons and should be re-tested.

Product Performance

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Precision: There are no more than one false negative and false positive judgment results within a batch; the color development of the test line and the control line between the three batch number test strips (cards) between batches should be uniform, and the color development between batches is not uniform. There is no more than one judgment result.

Stability: 2-30 ℃ unopened validity period of 2 years

Precautions for using the Canine Parvovirus Antigen Test Kit

1. Please read the instructions carefully before the test. The various reagents provided by this product are only for this test.
2. Products with expired or damaged aluminum foil bags cannot be used.
3. If the kit is taken out under low temperature storage, it needs to be returned to room temperature before opening, and the opened test card should be used as soon as possible to avoid moisture, and it should not exceed 1 hour.
4. Avoid touching the sample well on the test card and the chromatographic strip at the test window.
5. The waste after the test should be regarded as pollutants and properly disposed of according to relevant local regulations.

Test principle of the Canine Adenovirus Antigen Test Kit

The Canine Adenovirus Antigen Test Kit uses a rapid immunochromatographic assay to detect canine adenovirus antigens. You add a sample of your dog’s eye discharge, nasal fluid, or saliva to the spiking well of the test strip and move it along the chromatographic membrane with a colloidal gold-labeled anti-CAVt monoclonal antibody. If the CAV antigen is present in the sample, it will show red when bound to the antibody on the detection line. No color reaction is produced if the CAV antigen is not present in the sample.

The Canine Adenovirus Antigen Test Kit is able to detect Canine Adenovirus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only.

Intend Use

For rapid screening of Canine Adenovirus in canine eye secretions, nasal fluid, saliva and vomit. (CAV).

Components of the CAV-Ag Test Kit

Item NO.	Test Cassette	Diluent	Disposable Swab	Instruction
CY-G0011-1 (1 test)	1 pc	1 pc	1 pc	1 pc
CY-G0011-10 (10 tests)	10 pcs	10 pcs	10 pcs	1 pc
CY-G0011-25 (25 tests)	25 pcs	25 pcs	25 pcs	1 pc

Storage conditions and Validity Period:

Store at 2-30°C, valid for 24 months.

Sample Requirements

• This Canine Adenovirus Antigen Test Kit is only suitable for testing eye secretions, nasal fluids, and saliva samples from dogs.
• The sample for the test must be collected immediately, the sample should be put into the dilution solution immediately after sampling, and the diluted sample should be tested as soon as possible, within 1 hour.
• Diluted samples can be stored at 2-8°C for 24 hours and can be stored at -20°C for one month.

How to Use Canine Adenovirus Antigen Test Kit

1. Disposable swabs were used to collect samples of eye secretions, nasal fluids, and saliva from the dogs, and then the swabs were placed in the diluted solution and mixed and left to stand for 1 minute. Remove the swab, tighten the cap, and turn the sample tube upside down to mix.
2. Place the test strip on a flat, clean table.
3. Pipette 3 drops (approximately 80 μl) of sample onto the assay tape and wait for the test results.
4. Wait for 10-15 min at room temperature to read the test results. After 20 min, the test results are invalid.

Note: The above interpretation time is conducted at room temperature 15-30℃. If the temperature is lower than 15℃, the interpretation time should be delayed appropriately. If the temperature exceeds 30 ℃, the results should be read in advance.

Interpretation of Results

Negative: Only the C line appears as a red color.
Positive: Both T and C lines appear purplish red.
Invalid: No color appears in the C line.

NOTE: Invalid test results can be caused by a variety of reasons and should be re-tested.

Product Performance

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Stability: 2-30 ℃ unopened validity period of 2 years

Precautions for using the Canine Adenovirus Antigen Test Kit

• Please read the instructions carefully before testing. The Canine Adenovirus Antigen Test Kit is for this test only.
• Do not use it if the product has expired or the foil bag is damaged.
• If the Canine Influenza Virus Antigen Test Kit is removed from cryopreservation, it should be opened after returning to room temperature and used within 1 hour.
• Avoid contact with the sample wells and laminar strips on the test tape.

Test principle of Canine Parvovirus Antibody Test Kit

The kit is made by the principle of colloidal gold immunochromatography. After the sample is added to the sample hole, it moves along the chromatographic membrane together with the colloidal gold marker. If there is CPV antibody in the sample, the detection line will display purple-red. If there is no CPV in the sample Antibodies do not produce a color reaction.

The Canine Parvovirus test kit is able to detect Canine Parvovirus antigen concentration, demonstrating quantitative results. The test serves only as an aid to the diagnosis. For professional use only. And the quantitative results are trackable.

Intend Use

For rapid screening of Canine Parvovirus in canine whole blood, serum, or plasma (CPV).

Components of the CPV-Ab Test Kit

Item NO.	Test Cassette	Finger-stick Device	Assay Buffer	Finger-stick Device	Diluent	Instruction
CY-G007-1 (1 test)	1 pc	1 pc	1 pc	1 pc	1 pc	1 pc
CY-G007-10 (10 tests)	10 pcs	10 pcs	10 pcs	10 pcs	10 pcs	1 pc
CY-G007-25 (25 tests)	25 pcs	25 pcs	25 pcs	25 pcs	25 pcs	1 pc

Storage Conditions and Validity Period

Store at 2-30°C, valid for 24 months.

Sample Requirements

• This kit is only suitable for testing canine whole blood, serum or plasma samples.
• Test samples Collect fresh samples and should be tested as soon as possible within 1 hour.
• If not detected in time, whole blood, serum or plasma samples can be stored at 2-8°C for 3 days, and serum or plasma samples can be stored at -20°C for 1 month.

How to use the Canine Parvovirus Antibody Test Kit？

• Collect dog whole blood, serum or plasma samples, add the samples to the diluent after collection, tighten the lid, and mix by inversion.
• Tear open the aluminum foil bag of the test card, take out the test card, and place it on a flat and clean table.
• Pipette 3 drops (about 80 μl) of the diluted sample into the sample hole of the test card and wait for the test result.
• Wait 10-15 minutes at room temperature to interpret the test results, and the test results after more than 20 minutes are invalid.

Remarks: The above interpretation time is carried out at room temperature of 15-30°C. If the temperature is lower than 15°C, the interpretation time should be appropriately extended; if the temperature is higher than 30°C, the interpretation time should be appropriately shortened.

Interpretation of Results

Negative: Within the detection window, only the control line (C) has a purple-red band.

Positive: Within the detection window, both the test line (T) and the control line (C) appear purple-red bands.

Invalid: In the detection window, the control line (C) does not appear purple-red band.

• A negative test result simply means that there are no canine parvovirus antibodies in the test sample.
• The test result was positive surface test samples containing canine parvovirus antibodies.
• Invalid test results can be caused by a variety of reasons and should be re-tested.

Limitations of Detection Method

This kit can only detect whether there are canine parvovirus antibodies in the tested sample.
The test results of this kit are for clinical reference only and are not used as a basis for diagnosis.

Product Performance

Sensitivity ≥ 95%

Specificity ≥ 97.5%

Precision: There are no more than one false negative and false positive judgment results within a batch; the color development of the test line and the control line between the three batch number test strips (cards) between batches should be uniform, and the color development between batches is not uniform. There is no more than one judgment result.

Stability: 2-30 ℃ unopened validity period of 2 years

Precautions for using the Canine Parvovirus Antibody Test Kit

1. Please read the instructions carefully before the test. The various reagents provided by this product are only for this test.
2. Products with expired or damaged aluminum foil bags cannot be used.
3. If the kit is taken out under low-temperature storage, it needs to be returned to room temperature before opening, and the opened test card should be used as soon as possible to avoid moisture, and it should not exceed 1 hour.
4. Avoid touching the sample well on the test card and the chromatographic strip at the test window.
5. The waste after the test should be regarded as pollutants and properly disposed of according to relevant local regulations.

Test principle of the Canine Rotavirus Antigen Test Kit

This product uses a rapid immunochromatographic technique for the detection of canine rotavirus antigens in canine feces or vomit. If there is CRV antigen in the sample, it will specifically bind to the recombinant protein labeled with colloidal gold to form a complex that will move along the chromatographic membrane and be captured by the CRV antibody coated on the membrane, showing a wine red line on the detection line (T). If the CRV antigen is not present in the sample, no visible line is formed at the T position. No matter the result is negative or positive, the C line should be colored, otherwise the result is invalid.

Components of the Infectious CRV-Ag Test Kit

Component	CY-CRVAG-01 (1 test)	CY-CRVAG-10 (10 tests)	CY-CRVAG-25 (25 tests)
Test device in foil bag (including test card,pipette &desiccant)	1 pc	10 pcs	25 pcs
Extraction vial/cap (contains extraction buffer solution)	1 vial and cap	10 vials and caps	25 vials and caps
Sampling swab	1 pc	10 pcs	25 pcs
IFU	1 pc	1 pc	1 pc

Storage conditions and Validity Period:

Store at 2-30°C and be valid for 24 months.

Sample Requirements of Canine Rotavirus Antigen Test Kit

• Please refer to the instructions for use
• This kit is only suitable for testing feline whole blood or serum samples (hemolysis and hyperlipidemia samples cannot be used).
• Collect fresh samples and immediately put into the extraction buffer solution, which should be detected as soon as possible within 1 hour.
• If samples were not timely detected, store within 24 hours at 2-8°C and store within 1 month at -20°C.

Product Performance Indifelineors

1. Sensitivity ≥ 95%.
2. Specificity ≥ 97.5%.
3. Precision: no more than one false negative and false positive results within the batch; the test lines & control line (cards) between three lots shall be uniform, and no more than one time of color difference between lots.
4. Stability: valid for 24 month if unopened & store 2-30°C.

Interpretation of Results

Negative: In the test window, only one fuchsia-colored line appear next to “C”.
Positive: In the test window, two distinct colored lines appear next to “T” & “C” simultaneously.
Invalid: No color line visible next to “C”.

NOTE: Invalid test results can be caused by a variety of reasons and should be re-tested.