HCY™ Klentaq DNA Polymerase Kit

HCY™ Klentaq DNA Polymerase Kit

With a 278 amino acid deletion at the N-terminus of KlenTaq DNA Polymerase, the truncated Klentaq enzyme has higher fidelity and thermal stability compared to wild-type Taq enzymes. The enzyme retained the DNA polymerase activity of DNA polymerase I and 3’→5′ nucleic acid exonuclease activity, and lacked 5’→3′ nucleic acid exonuclease activity. The product carries an A base at the 3′ end and can be directly cloned with TA vector.

Source

HCY™ KlenTaq DNA Polymerase is Recombinant KlenTaq DNA Polymerase, which is genetically modified by mutation and recombination, and is obtained by expression purification in E. coli.

Uses

• Conventional PCR, high-fidelity PCR, genotyping and primer extension analysis, etc.
• HCY™ KlenTaq enzyme can amplify DNA fragments up to 5 kb in length. It is usually suitable for amplification of DNA fragments up to 3 kb in length.
• Most PCR products amplified with this product have an “A” base at the 3′ end and can therefore be cloned directly into the T-Vector.

Activation Definition

Using activated salmon sperm DNA as template/primer, the activity of 10 nmol of whole nucleotides ingested as acid insoluble was defined as 1 unit of activity (U) at 74°C for 30 min.

Performance of HCY™ Klentaq DNA Polymerase

Quality Control

• SDS-PAGE electrophoretic purity greater than 98%;
• Functional test: sensitivity, specificity, reproducibility of PCR;
• No exogenous nuclease activity, no exogenous endonuclease, and exonuclease contamination;
• No expression of host nucleic acid residue.

Precautions: This product is for scientific research only and cannot be used for medical or diagnostic procedures in humans or animals or as food, cosmetics or household products. Without written permission, authorization, or approval, this product may not be manufactured, licensed for sale, sold, imported or exported, or use all related patents and trademarks of the product.

Packing List

Cat. No.	Product Name	Package
E0080P01	HCY™ KlenTaq DNA Polymerase	500U/tube
E0080B01	5×HCY™ KlenTaq Buffer (with Mg2+)	1ml/tube (2 tubes)
–	Manual	1pc

Storage Conditions

For long term storage, please store at -20℃ away from light. If frequent use is required, it can be divided and stored at 4℃ to avoid repeated freezing and thawing. After thawing, it should be mixed well to avoid generating a lot of air bubbles.