Powdered Minimum Essential Medium (MEM)

MEM can be used for the culture of a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes.etc. MEM cell culture medium does not contain  proteins, lipids or growth factors. So it usually requires to add 10% fetal bovine serum (FBS) as nutrient supplementation. MEM contains Earle’s Balanced Salts for a CO2 incubator or Hanks’ Balanced Salts for a CO2-free incubator.
For a wide range of cell culture applications, we offer modified MEM media with various components, as well as MEM media with customizable components.

Ingredients of Powdered Minimum Essential Medium (MEM)

P/N	Specification	Ingredient Description
HCY-BCMP01001A	1L×1 bag	+: L-Glutamine、HEPES、Phenol red、Earle’s salt -: sodium pyruvate、sodium bicarbonate
HCY-BCMP01001B	10L×1 bottle	+: L-Glutamine、HEPES、Phenol red、Earle’s salt -: sodium pyruvate、sodium bicarbonate
HCY-BCMP01002A	1L×1 bag	+: L-Glutamine、NEAA、Phenol red、Earle’s salt -: HEPES、sodium pyruvate、sodium bicarbonate
HCY-BCMP01002B	10L×1 bottle	+: L-Glutamine、NEAA、Phenol red、Earle’s salt -: HEPES、sodium pyruvate、sodium bicarbonate
HCY-BCMP01003A	1L×1 bag	+: L-Glutamine、Phenol red、Earle’s salt –: HEPES、sodium pyruvate、sodium bicarbonate
HCY-BCMP01003B	10L×1 bottle	+: L-Glutamine、Phenol red、Earle’s salt -: HEPES、sodium pyruvate、sodium bicarbonate

This MEM dry powder medium needs to be supplemented with sodium bicarbonate, adjusted pH and filtered when preparing. The detailed formulation method is as follows:

1. Add 950 mL of distilled water to a mixing vessel as close to final volume as possible.
2. Add dry medium to room temperature (15°C to 30°C) water and stir gently. Do not heat water.
3. Rinse the inside of the pack to remove any residual powder.
4. Add 29.3 mL of 75 g/L NaHCO3 solution to the medium.
5. Adjust the pH to 0.2 to 0.3 units below the desired final working pH by slowly adding and stirring 1 N NaOH or 1 N HCl. The pH may rise by 0.1 to 0.3 units after filtration. The recommended working pH is 7.1-7.4.
6. Adjust final volume with distilled water.
7. Immediately process the medium through the membrane filtration function of a 0.2 μm filter into a sterile container using a positive pressure system.