FTA Cards, short for Flinders Technology Association Cards, represent a groundbreaking innovation in biological sample management, redefining the way we collect, preserve, and transport biological specimens for DNA and RNA analysis. These chemically treated filter papers have become an indispensable tool across various scientific and medical fields, offering unparalleled convenience, reliability, and efficiency.​

Working Principle​
At the core of FTA Cards’ functionality lies their unique composition of cotton-based cellulose paper impregnated with specific chemicals. When a biological sample, such as blood, saliva, or tissue cells, is applied to the FTA Card, these chemicals initiate a series of reactions. They cause cell lysis and protein denaturation, while simultaneously immobilizing nucleic acids within the card’s matrix. This process effectively protects the nucleic acids from degradation by ultraviolet light, oxidation, and nucleases, ensuring their integrity for downstream analysis.​

Usage​
Using FTA Cards is a straightforward process. Simply apply the biological sample to the designated area on the card. Once the sample dries, the nucleic acids are stably bound to the card. For subsequent nucleic acid analysis, a small section of the card containing the sample is cut out and subjected to a simple purification procedure. The purified nucleic acids can then be used in a wide range of downstream experiments, including PCR, sequencing, and SNP analysis.​

Types​
FTA Cards come in various types to meet different sample collection and storage needs. The FTA Classic Card features 4 sample areas and can store up to 500µl of whole blood or 100µl of plant homogenate. The FTA Mini Card has 2 sample areas and can hold up to 250µl of whole blood or 50µl of plant homogenate. The FTA Micro Card, with 1 sample area, is designed to store up to 125µl of whole blood or 25µl of plant homogenate. The FTA GeneCard, equipped with 3 sample areas, is compatible with a variety of automated liquid handling systems. Finally, the FTA Elute Micro Card, which has 4 sample areas, is ideal for applications such as STR analysis, sequencing, and real-time PCR.​

Advantages​
One of the most significant advantages of FTA Cards is their ability to store and transport biological samples at room temperature. This eliminates the need for costly and cumbersome cold storage facilities, making sample handling more convenient and cost-effective. Additionally, samples stored on FTA Cards can be preserved for extended periods. Genomic DNA on FTA Cards can remain viable for PCR amplification for over 17.5 years at room temperature. FTA Cards also rapidly inactivate infectious pathogens in samples, reducing the risk of disease transmission and often eliminating the need for import licenses. Moreover, their simple operation – just drop the sample on the card and let it dry – makes them accessible to users with varying levels of technical expertise.​
Applications​
In the field of molecular biology, FTA Cards are widely used for whole genome sequencing, genomic research, and plasmid screening. In forensic science, they play a crucial role in STR analysis, animal identification, and transgenic identification. In the medical field, they are applied in transfusion medicine and drug development. In agriculture, they are used for food testing and other agricultural inspections.​
In conclusion, FTA Cards offer a comprehensive solution for biological sample management. Their innovative design, ease of use, and wide range of applications make them an essential tool for researchers, forensic scientists, medical professionals, and agricultural experts alike. Whether you’re working on a large – scale genomic project, conducting forensic investigations, or developing new medical treatments, FTA Cards provide a reliable and efficient way to handle biological samples. Embrace the future of biological sample management with FTA Cards and experience the difference in your research and applications.​