Phosphate Buffered Saline Buffer, PBS

Specification of  Phosphate Buffered Saline Buffer

Shelf Life:	24 months
Endotoxin Content:	< 0.25 EU/mL
Storage Temperature:	Ambient Temperature
Shipping Temperature:	Ambient Temperature
Mycoplasma:	Tested Negative

Component of  Phosphate Buffered Saline Buffer

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g/mol)	8 g	0.137 M
Potassium Chloride (mw: 74.55 g/mol)	0.2 g	0.0027 M
Sodium Phosphate Dibasic (mw: 141.96 g/mol)	1.44 g	0.01 M
Potassium Phosphate Monobasic (mw: 136.09 g/mol)	0.245 g	0.0018 M

Applications

PBS is used in various laboratory techniques, including immunodetection, microarraying, biochemistry, purification, and cell culture. It can be supplemented by a variety of additives (chelatants such as EDTA, detergents such a Tween20, proteins such as BSA, bacteriostatic such as azide, protease inhibitors such as AEBSF,…). See related products below for other PBS products including additives.

Note:

Phosphate buffer should not be used in assays where competition for phosphate groups, or complex formation
with a metal ion is essential for the enzyme activation. Phosphate ions will inhibit carboxypeptidase, carboxylase,
urease, muscle diaminase, formase and phosphoglucomutase.

Related Products

Product	Cat No.
* PBS pH7.4, tablets for 100-200-500-1000ml,	Cat No.:CY-PB-T0
* PBS pH7.4 sterile solutions	Cat No.:CY-PB-TS
* PBS for saturation in immunodetection techniques (BSA or Tween or Milk PBS blends)	Cat No.:CY-PB-SI
PBS with Non-Fat Powdered Milk 3%, pH 7.5 , powder packs	Cat No.:CY-PB-NP
PBS with BSA 1%, pH 7.5, powder packs	Cat No.:CY-PB-BS
PBS with Tween® 0.05%, pH 7.5 , powder packs #GS4250	Cat No.:CY-PB-TW
PBS with Calcium and magnesium: Dulbecco’s PBS buffer	Cat No.:CY-PB-CM