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HCY™ Candidatus Mycoplasma turicensis (CMt) Real-time PCR Detection Kit (Lyophilized Bead)

Huachenyang — Mon, 12 Apr 2021 09:43:00 +0000

HCY™ Candidatus Mycoplasma turicensis (CMt) Real-time PCR Detection Kit (Lyophilized Bead)

Cat. No.: CY-CMT-LM10

CMt Mycoplasma haemofelis PCR Detection Kit

HCY™ Candidatus Mycoplasma haemminutum (Cmt) PCR Detection Kit is a fluorescent quantitative assay for the detection of feline mycoplasma hematophilum (CMt) in blood and other samples.

Specific primers and Taqman probe are designed with the conserved gene of CMt type feline hemophilic mycoplasma, and amplified by fluorescence quantitative PCR instrument, so as to realize the detection of CMt type feline hemophilic mycoplasma.

Meanwhile, this kit uses the cat RPS7 gene as the internal reference gene, which meets the requirements of indoor quality control in the whole process from sampling, sample purification to on-line testing.

＋86-755-2739 3226 info@chenyanglobal.com

Email us

Request Quote

PRODUCT DETAIL

INQUIRY

HCY™ Candidatus Mycoplasma turicensis (CMt) Real-time PCR Detection Kit

Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
Easy to use, no need to prepare reagents in advance;
The minimum detection limit is 2 copies/Test;
Clinical sensitivity 100%, specificity 99.15%;
Add internal standard to prevent false negative results and facilitate indoor quality control.

Quality by Design

The typing design was based on three species of Mycoplasma haemophilum (Mhf, CMhm and CMt), and 828 sequences of feline Mycoplasma haemophilum included in NCBI GenBank were compared for homology, and an evolutionary tree was constructed and analyzed.

This test kit is designed and developed based on the above gene sequences of endemic pathogens in different regions of the world, which fully ensures the accuracy and validity of the test. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.

LoD and PCR Efficiency

Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 2 copies/Test. Calculation of the experimental data set yielded R²=0.999 and the amplification efficiency E=97.87%.

Amplification Curve

Amplification Efficiency

Sensitivity and Specificity

The HCY™ Mycoplasma haemofelis (CMt) PCR Detection Kit was compared to similar commercially available products and tested 127 real clinical samples, of which the sensitivity was 100% and the specificity 99.15%.


		Reference Product		Total
		Negative	Positive	Total
HCY™ PCR Test Kit	Positive	1	9	10
HCY™ PCR Test Kit	Negative	117	0	117
Total		118	9	127


	Estimated Value	95% Confidence Interval
Sensitivity	100%	62.88%~100%
Specificity	99.15%	94.68%~100%

Cross-reactivity

The HCY™ Mycoplasma haemofelis PCR Detection Kit is used for Bartonella, Borrelia Burgdorferi, Toxoplasmosis, Leptospira, Bordetella bronchiseptica, Feline calicivirus, Feline herpesvirus, Feline Parvovirus, Chlamydia felis, Feline Coronavirus and Candidatus Mycoplasma turicensis, Canine parainfluenza Virus), Canine Coronavirus, Canine Herpesvirus, Canine Distemper virus, Canine Parvovirus, no cross-reactivity.

“HCY, Health care for you” is our forever mission. We dedicate to offering safe & reliable products and medical services with our global creditable partners. HCY has already supplied to WHO, MAYO clinic, MGI, DDC, Yale University, Qorvo, Quanterix, Thomas Scientific, SD biosensor, Cardinal Health, Cleveland Clinic, Mars Petcare & LumiraDx, etc. in the past years.

You can request a quote or submit the order.

We will reply to you via email in 24 hours

文章HCY™ Candidatus Mycoplasma turicensis (CMt) Real-time PCR Detection Kit (Lyophilized Bead)最初出现于A professional supplier of swabs.

HCY™ Candidatus Mycoplasma haemminutum (Mhf) Real-time PCR Detection Kit (Lyophilized Bead)

Huachenyang — Tue, 12 Jan 2021 07:58:00 +0000

HCY™ Candidatus Mycoplasma haemminutum (Mhf) Real-time PCR Detection Kit (Lyophilized Bead)

Cat. No.: CY-MHF-LM10

Mhf Mycoplasma haemofelis PCR Detection Kit

HCY™ Candidatus Mycoplasma haemminutum (Mhf) PCR Detection Kit is a fluorescent quantitative assay for the detection of Mycoplasma feline hemophilus (Mhf) in blood and other samples.

Specific primers and Taqman probe are designed with the conserved gene of Mhf type feline hemophilic mycoplasma, and amplified by fluorescence quantitative PCR instrument.

Meanwhile, this kit uses the cat RPS7 gene as the internal reference gene, which meets the requirements of the whole process of indoor quality control from sampling, sample purification to on-line testing.

＋86-755-2739 3226 info@chenyanglobal.com

Email us

Request Quote

PRODUCT DETAIL

INQUIRY

HCY™ Candidatus Mycoplasma haemminutum (Mhf) PCR Detection Kit

Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
Easy to use, no need to prepare reagents in advance;
The minimum detection limit is 1 copies/Test;
Clinical sensitivity 100%, specificity 98.25%;
Add internal standard to prevent false negative results and facilitate indoor quality control.

Quality by Design

Candidatus Mycoplasma haemminutum (Mhf) PCR Detection Kit is designed and developed based on the above gene sequences of endemic pathogens in different regions of the world, which fully ensures the accuracy and validity of the test. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.

LoD and PCR Efficiency

Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 1 copies/Test. Calculation of the experimental data set yielded R²=0.998 and the amplification efficiency E=94.54%.

Amplification Curve

Amplification Efficiency

Sensitivity and Specificity

The HCY™ Mycoplasma haemofelis PCR Detection Kit was compared to similar commercially available products and tested 127 real clinical samples, of which the sensitivity was 100% and the specificity 98.25%.


		Reference Product		Total
		Negative	Positive	Total
HCY™ PCR Test Kit	Positive	2	113	15
HCY™ PCR Test Kit	Negative	112	0	112
Total		114	15	127


	Estimated Value	95% Confidence Interval
Sensitivity	100%	71.66%~100%
Specificity	100%	93.18%~100%

Cross-reactivity

You can request a quote or submit the order.

We will reply to you via email in 24 hours

文章HCY™ Candidatus Mycoplasma haemminutum (Mhf) Real-time PCR Detection Kit (Lyophilized Bead)最初出现于A professional supplier of swabs.