HCY™ Feline Calicivirus PCR Detection Kit is a fluorescent quantitative assay for the detection of feline cupripoxvirus in samples such as buccal s...View details
HCY™ Candidatus Mycoplasma haemminutum (Mhf) PCR Detection Kit is a fluorescent quantitative assay for the detection of Mycoplasma feline hemophilus (Mhf) in blood and other samples. Specific primers and Taqman probe are designed with the conserved gene of Mhf type feline hemophilic mycoplasma, and amplified by fluorescence quantitative PCR instrument. Meanwhile, this kit uses the cat RPS7 gene as the internal reference gene, which meets the requirements of the whole process of indoor quality control from sampling, sample purification to on-line testing.
HCY™ Candidatus Mycoplasma haemminutum (Mhf) PCR Detection Kit
- Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
- The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
- Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
- Easy to use, no need to prepare reagents in advance;
- The minimum detection limit is 1 copies/Test;
- Clinical sensitivity 100%, specificity 98.25%;
- Add internal standard to prevent false negative results and facilitate indoor quality control.
Quality by Design
The typing design was based on three species of Mycoplasma haemophilum (Mhf, CMhm and CMt), and 828 sequences of feline Mycoplasma haemophilum included in NCBI GenBank were compared for homology, and an evolutionary tree was constructed and analyzed.
Candidatus Mycoplasma haemminutum (Mhf) PCR Detection Kit is designed and developed based on the above gene sequences of endemic pathogens in different regions of the world, which fully ensures the accuracy and validity of the test. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.
LoD and PCR Efficiency
Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 1 copies/Test. Calculation of the experimental data set yielded R2=0.998 and the amplification efficiency E=94.54%.
Sensitivity and Specificity
The HCY™ Mycoplasma haemofelis PCR Detection Kit was compared to similar commercially available products and tested 127 real clinical samples, of which the sensitivity was 100% and the specificity 98.25%.
|HCY™ PCR Test Kit||Positive||2||113||15|
|Estimated Value||95% Confidence Interval|
The HCY™ Mycoplasma haemofelis PCR Detection Kit is used for Bartonella, Borrelia Burgdorferi, Toxoplasmosis, Leptospira, Bordetella bronchiseptica, Feline calicivirus, Feline herpesvirus, Feline Parvovirus, Chlamydia felis, Feline Coronavirus and Candidatus Mycoplasma turicensis, Canine parainfluenza Virus), Canine Coronavirus, Canine Herpesvirus, Canine Distemper virus, Canine Parvovirus, no cross-reactivity.
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