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HCY™ Canine coronavirus PCR Detection Kit adopts the fluorescence quantitative detection method for the detection of Canine Coronavirus in feces, anal swabs, etc. Specific primers and Taqman probe are designed with the conserved genes of Canine Coronavirus, which are amplified by the fluorescence quantitative PCR instrument. Meanwhile, this kit uses GAPDH gene as the internal reference gene, which meets the requirements of indoor quality control in the whole process from sampling, sample purification to on-line testing.
HCY™ Canine Coronavirus PCR Detection Kit
- Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
- The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
- Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
- Easy to use, no need to prepare reagents in advance;
- The minimum detection limit is 4 copies/Test;
- Clinical sensitivity 100%, specificity 100%;
- Add internal standard to prevent false negative results and facilitate indoor quality control.
Quality by Design
Canine Coronavirus (CCoV) has both intestinal and respiratory subtypes, CCOV and CRCV, and CCoV-Ⅰ and CCoV-Ⅱ, CCoV-Ⅱ is divided into two genotypes, CCoV-Ⅱa and CCoV-Ⅱb. We compared the 1508 CCOV sequences in NCBI GenBank, 440 sequences in China, and the reference sequence CD19_21010626 (OQ623693), and constructed an evolutionary tree for homology analysis. 21010626 (OQ623693) were compared, homology comparison was performed and evolutionary tree analysis was constructed.
HCY™ Canine coronavirus PCR Detection Kit is is designed and developed based on the above gene sequences of endemic pathogens in different regions of the world, which fully ensures the accuracy and validity of the test. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.
LoD and PCR Efficiency
Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 4 copies/Test. Calculation of the experimental data set yielded R2=0.999 and the amplification efficiency E=99.2%.
Sensitivity and Specificity
The HCY™ Canine coronavirus PCR Detection Kit was compared to similar commercially available products and tested 135 real clinical samples, of which the sensitivity was 100% and the specificity 100%.
|HCY™ PCR Test Kit||Positive||0||22||18|
|Estimated Value||95% Confidence Interval|
The HCY™ Canine coronavirus PCR Detection Kit was validated against Bartonella, Borrelia Burgdorferi, Toxoplasmosis, Leptospira, Bordetella bronchiseptica, Feline Calicivirus, Feline coronavirus, Feline herpesvirus, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis, Chlamydia Felis, Parainfluenza Virus, Canine Herpesvirus, Canine Distempervirus, and Canine Parvovirus, all of which did not cross-react.
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