HCY™ Canine Parainfluenza Virus PCR Detection Kit is a fluorescent quantitative assay for the detection of Canine parainfluenza Virus (CPIV) in pla...View details
HCY™ Feline Coronavirus PCR Detection Kit is a fluorescent quantitative assay for the detection of feline coronavirus in samples such as ocular and nasal secretions, feces, ascites and viscera. Specific primers and Taqman probes are designed with the conserved genes of feline coronavirus and amplified by fluorescent quantitative PCR. Meanwhile, the kit uses the cat RPS7 gene as the internal reference gene, which meets the requirements of indoor quality control in the whole process from sampling, sample purification to on-line testing.
HCY™ Feline Coronavirus PCR Detection Kit
- Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
- The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
- Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
- Easy to use, no need to prepare reagents in advance;
- The minimum detection limit is 5 copies/Test;
- Clinical sensitivity 100%, specificity 99.11%;
- Add internal standard to prevent false negative results and facilitate indoor quality control.
Quality by Design
FIP virus strain reference sequence (NC_002306.3), and China, Japan, Italy together included 147 for comparison, the combined FECV sequence 3580 gene sequences (NC_002306.3) gene comparison results analysis, for the construction of an evolutionary tree to confirm the existence of multiple subspecies of FECV + FIPV homology in the region of China (NC_ 002306.3) for homology comparison and constructing evolutionary tree analysis.
HCY™ Feline Coronavirus PCR Detection Kit is designed and developed based on the gene sequences of endemic pathogens in different regions of the world, as mentioned above, which fully ensures the accuracy and validity of the detection. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.
LoD and PCR Efficiency
Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 5 copies/Test. Calculation of the experimental data set yielded R2=0.998 and the amplification efficiency E=95.9%.
Sensitivity and Specificity
The HCY™ Feline Coronavirus PCR Detection Kit was compared to similar commercially available products and tested 127 real clinical samples, of which the sensitivity was 100% and the specificity 99.11%.
|HCY™ PCR Test Kit||Positive||1||15||16|
|Estimated Value||95% Confidence Interval|
The HCY™ Feline Coronavirus PCR Detection Kit is used for Bartonella, Borrelia Burgdorferi, Toxoplasmosis, Leptospira, Bordetella bronchiseptica, Feline calicivirus, Feline herpesvirus, Feline Parvovirus, Chlamydia felis, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis, Canine parainfluenza Virus), Canine Coronavirus, Canine Herpesvirus, Canine Distemper virus, Canine Parvovirus, no cross-reactivity.
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