HCY™ Canine Parainfluenza Virus PCR Detection Kit is a fluorescent quantitative assay for the detection of Canine parainfluenza Virus (CPIV) in pla...View details
HCY™ Chlamydia felis PCR Detection Kit is a fluorescent quantitative assay for the detection of feline chlamydia in samples such as eyelid secretions, conjunctival secretions and nasal secretions. Specific primers and Taqman probe are designed with the conserved gene of Cf and amplified by fluorescent quantitative PCR instrument. Meanwhile, the kit uses the cat RPS7 gene as the internal reference gene, which meets the requirements of the whole process of indoor quality control from sampling, sample purification to on-line testing.
HCY™ Chlamydia Felis PCR Detection Kit
- Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
- The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
- Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
- Easy to use, no need to prepare reagents in advance;
- The minimum detection limit is 1 copies/Test;
- Clinical sensitivity 100%, specificity 100%;
- Add internal standard to prevent false negative results and facilitate indoor quality control.
Quality by Design
A total of 317 feline Chlamydia sequences included in NCBI GenBank were compared with NC_007900.1 as the reference sequence for homology comparison and construction of evolutionary tree analysis.
HCY™ Chlamydia felis PCR Detection Kit is designed and developed based on the above gene sequences of endemic pathogens in different regions of the world, which fully ensures the accuracy and validity of the test. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.
LoD and PCR Efficiency
Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 1 copies/Test. Calculation of the experimental data set yielded R2=0.999 and the amplification efficiency E=96.29%.
Sensitivity and Specificity
The HCY™ Chlamydia felis PCR Detection Kit was compared to similar commercially available products and tested 127 real clinical samples, of which the sensitivity was 100% and the specificity 100%.
|HCY™ PCR Test Kit||Positive||0||10||10|
|Estimated Value||95% Confidence Interval|
The HCY™ Chlamydia Felis PCR Detection Kit was validated against Bartonella, Borrelia Burgdorferi, Toxoplasmosis, Leptospira, Bordetella bronchiseptica, Feline Calicivirus, Feline coronavirus, Feline herpesvirus, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis, Canine parainfluenza Virus, Canine Coronavirus, Canine Herpesvirus, Canine Distempervirus, and Canine Parvovirus, all of which did not cross-react.
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