HCY™ Feline Calicivirus (FCV) Real-time PCR Detection Kit (Lyophilized Bead)

HCY™ Feline calicivirus PCR Detection Kit

• Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
• The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
• Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
• Easy to use, no need to prepare reagents in advance;
• The minimum detection limit is 20 copies/Test;
• Clinical sensitivity 100%, specificity 98.06%;
• Add internal standard to prevent false negative results and facilitate indoor quality control.

Quality by Design

The kit (lyophilized microspheres) is a fluorescent quantitative assay for the detection of feline cupripoxvirus in samples such as buccal swabs, conjunctival swabs, nasal swabs, blood, and organs. Specific primers and Taqman probes are designed with the conserved genes of cat cupulovirus and amplified by fluorescent quantitative PCR instrument. Meanwhile, this kit uses the cat RPS7 gene as the internal reference gene, which meets the requirements of indoor quality control in the whole process from sampling, sample purification to on-line testing.

HCY™ Feline calicivirus PCR Detection Kit is designed and developed based on the gene sequences of endemic pathogens in different regions of the world, as mentioned above, which fully ensures the accuracy and validity of the detection. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.

LoD and PCR Efficiency

Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 20 copies/Test. Calculation of the experimental data set yielded R²=0.998 and the amplification efficiency E=93.727%.

Sensitivity and Specificity

The HCY™ Feline calicivirus PCR Detection Kit was compared to similar commercially available products and tested 127 real clinical samples, of which the sensitivity was 100% and the specificity 98.06%.

Cross-reactivity

The HCY™ Feline calicivirus PCR Detection Kit is used for Bartonella, Borrelia Burgdorferi, Toxoplasmosis, Leptospira, Bordetella bronchiseptica, Feline coronavirus, Feline herpesvirus, Feline Parvovirus, Chlamydia felis, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis, Canine parainfluenza Virus), Canine Coronavirus, Canine Herpesvirus, Canine Distemper virus, Canine Parvovirus, no cross-reactivity.