HCY™ Chlamydia felis PCR Detection Kit is a fluorescent quantitative assay for the detection of feline chlamydia in samples such as eyelid secretio...View details
HCY™ Feline Parvovirus PCR Detection Kit is a fluorescent probe method for the detection of feline microvirus in feces, serum, blood and other samples. Specific primers and Taqman probe are designed with the conserved genes of Feline Tiny Virus and amplified by fluorescent quantitative PCR instrument. Meanwhile, this kit uses the cat RPS7 gene as the internal reference gene, which meets the requirements of indoor quality control in the whole process from sampling, sample purification to on-line testing.
HCY™ Feline parvovirus PCR Detection Kit
- Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
- The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
- Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
- Easy to use, no need to prepare reagents in advance;
- The minimum detection limit is 10 copies/Test;
- Clinical sensitivity 100%, specificity 98.19%;
- Add internal standard to prevent false negative results and facilitate indoor quality control.
Quality by Design
The 981 FPV sequences in NCBI GenBank and 356 reference sequences (NC_001539.1) from China were compared for homology, and an evolutionary tree was constructed.
HCY™ Feline Parvovirus PCR Detection Kit is designed and developed based on the above gene sequences of endemic pathogens in different regions of the world, which fully ensures the accuracy and validity of the test. Meanwhile, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.
LoD and PCR Efficiency
Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 10 copies/Test. Calculation of the experimental data set yielded R2=0.995 and the amplification efficiency E=98.71%.
Sensitivity and Specificity
The HCY™ Feline Parvovirus PCR Detection Kit was compared to similar commercially available products and tested 127 real clinical samples, of which the sensitivity was 100% and the specificity 98.25%.
|HCY™ PCR Test Kit||Positive||2||16||18|
|Estimated Value||95% Confidence Interval|
HCY™ Feline Parvovirus PCR Detection Kit was validated against Bartonella, Borrelia Burgdorferi, Toxoplasmosis, Leptospira, Bordetella bronchiseptica, Feline Calicivirus, Feline coronavirus, Feline herpesvirus, Chlamydia felis, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis, Canine parainfluenza Virus, Canine Coronavirus, Canine Herpesvirus, Canine Distempervirus, and Canine Parvovirus, all of which did not cross-react.
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