HCY™ Borrelia Burgdorferi (BBu) Real-time PCR Detection Kit (Lyophilized Bead)

HCY™ Borrelia Burgdorferi Real-time PCR Detection Kit

• Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
• The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
• Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
• Easy to use, no need to prepare reagents in advance;
• The minimum detection limit is 50 copies/Test;
• Clinical sensitivity 100%, specificity 100%;
• Add internal standard to prevent false negative results and facilitate indoor quality control.

Quality by Design

The sequences of B. garinii (NC_017804.1), B. burgdorferi sensu stricto (CP002950.1), B. bissettii (CP002761.1), B. afzelii (NZ_JALGSO010000002.1), B.valaisian (NC_012177.1) together 49 sequences were aligned for homology comparison and constructed for evolutionary tree analysis.

HCY™ Borrelia Burgdorferi PCR Detection Kit is designed and developed based on the gene sequences of endemic pathogens in different regions of the world, as mentioned above, which fully ensures the accuracy and validity of the detection. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.

LoD and PCR Efficiency

Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 50 copies/Test. Calculation of the experimental data set yielded R2=0.9939 and the amplification efficiency E=105.39%.

Sensitivity and Specificity

The HCY™ Feline herpesvirus Real-time PCR Detection Kit was compared to similar commercially available products and tested 262 real clinical samples, of which the sensitivity was 100% and the specificity 100%.

Cross-reactivity

The HCY™ Borrelia Burgdorferi Real-time PCR Detection Kit has been validated for the detection of Feline herpesvirus, Bartonella, Toxoplasmosis, Leptospira, Bordetella bronchiseptica Feline Calicivirus, Feline coronavirus, Feline Parvovirus, Chlamydia felis, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma haemominutum. Candidatus Mycoplasma turicensis, Canine parainfluenza Virus, Canine Coronavirus, Canine Herpesvirus, Canine Distempervirus, and Canine Parvovirus. None of them cross-reacted.