HCY™ Parainfluenza Virus (CPIV) Real-time PCR Detection Kit (Lyophilized Bead)

HCY™ Canine Parainfluenza Virus PCR Detection Kit

• Designed based on the gene sequences of pathogens prevalent in various regions of the world, fully guaranteeing the accuracy and validity of the test;
• The primers are designed with special bases to reduce the risk of missed detection due to gene mutation;
• Adopting pre-filled freeze-dried microsphere technology for transportation and storage at room temperature;
• Easy to use, no need to prepare reagents in advance;
• The minimum detection limit is 1 copies/Test;
• Clinical sensitivity 100%, specificity 99.11%;
• Add internal standard to prevent false negative results and facilitate indoor quality control.

Quality by Design

Eight of the 42 CPIV sequences from China included in NCBI GenBank were compared for homology, and an evolutionary tree was constructed using the Chinese region (MT124463.1) as the reference sequence.

HCY™ Canine Parainfluenza Virus PCR Detection Kit is designed and developed based on the above gene sequences of endemic pathogens in different regions of the world, fully ensuring the test’s accuracy and validity. At the same time, special bases are introduced in the primer sequence synthesis process to reduce the risk of missed detection due to gene mutation.

LoD and PCR Efficiency

Using real clinical samples, the assay was performed after serial gradient dilution, and the results showed good linear characteristics, and the calculated copy number was as low as 10 copies/Test. Calculation of the experimental data set yielded R²=0.9987 and the amplification efficiency E=94.05%.

Sensitivity and Specificity

The HCY™ Canine Parainfluenza Virus PCR Detection Kit was compared to similar commercially available products and tested 135 real clinical samples, of which the sensitivity was 100% and the specificity 99.11%.

Cross-reactivity

The HCY™ Canine Parainfluenza Virus PCR Detection Kit was validated against Bartonella, Borrelia Burgdorferi, Toxoplasmosis, Leptospira, Bordetella bronchiseptica, Feline Calicivirus, Feline coronavirus, Feline herpesvirus, Mycoplasma haemofelis, Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis, Chlamydia Felis, Canine Coronavirus, Canine Herpesvirus, Canine Distempervirus, and Canine Parvovirus, all of which did not cross-react.