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Phosphate Buffered Saline Buffer, PBS

Phosphate Buffered Saline Buffer, PBS

Transport Medium & Swab
Buffer SolutionpbsPhosphate Buffered Saline

PBS (Phosphate Buffered Saline) (1X, pH 7.4) Preparation and Recipe
PBS is an isotonic buffer frequently used in biological applications, such as washing cells, transportation of tissues, and dilutions. PBS closely mimics the pH, osmolarity, and ion concentrations of the human body. Since it is nontoxic to cells, it is extensively used for cell container rinsing and other preparations that might leave a residue. It is simple to prepare and has a good shelf life, but it will precipitate in the presence of zinc ions.

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Specification of  Phosphate Buffered Saline Buffer

Shelf Life:24 months
Endotoxin Content:< 0.25 EU/mL
Storage Temperature:Ambient Temperature
Shipping Temperature:Ambient Temperature
Mycoplasma:Tested Negative

Component of  Phosphate Buffered Saline Buffer

ComponentAmountConcentration
Sodium chloride (mw: 58.44 g/mol)8 g0.137 M
Potassium Chloride (mw: 74.55 g/mol)0.2 g0.0027 M
Sodium Phosphate Dibasic (mw: 141.96 g/mol)1.44 g0.01 M
Potassium Phosphate Monobasic (mw: 136.09 g/mol)0.245 g0.0018 M

Applications

PBS is used in various laboratory techniques, including immunodetection, microarraying, biochemistry, purification, and cell culture. It can be supplemented by a variety of additives (chelatants such as EDTA, detergents such a Tween20, proteins such as BSA, bacteriostatic such as azide, protease inhibitors such as AEBSF,…). See related products below for other PBS products including additives.

Note:

Phosphate buffer should not be used in assays where competition for phosphate groups, or complex formation
with a metal ion is essential for the enzyme activation. Phosphate ions will inhibit carboxypeptidase, carboxylase,
urease, muscle diaminase, formase and phosphoglucomutase.

Related Products

ProductCat No.
* PBS pH7.4, tablets for 100-200-500-1000ml, Cat No.:CY-PB-T0
* PBS pH7.4 sterile solutionsCat No.:CY-PB-TS
* PBS for saturation in immunodetection techniques (BSA or Tween or Milk PBS blends)Cat No.:CY-PB-SI
PBS with Non-Fat Powdered Milk 3%, pH 7.5 , powder packs Cat No.:CY-PB-NP
PBS with BSA 1%, pH 7.5, powder packs Cat No.:CY-PB-BS
PBS with Tween® 0.05%, pH 7.5 , powder packs #GS4250 Cat No.:CY-PB-TW
PBS with Calcium and magnesium: Dulbecco’s PBS bufferCat No.:CY-PB-CM

“HCY, Health care for you” is our forever mission. We dedicate to offering safe & reliable products and medical services with our global creditable partners. HCY has already supplied to WHO, MAYO clinic, MGI, DDC, Yale University, Qorvo, Quanterix, Thomas Scientific, SD biosensor, Cardinal Health, Cleveland Clinic, Mars Petcare & LumiraDx, etc. in the past years.

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