4 Keys Technical Indicators of Virus Transport Medium (VTM)
4332The preservation effect, limit of detection (LOD), microbial index, inactivation rate are the key indicators for determining the precision and sens...
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Virus samples are usually not tested immediately after collection; the collected samples are stored and transported. Since the virus itself will quickly lyse in vitro and thus affect the results of subsequent testing, virus transport media are required to preserve and transport the virus.
Virus samples are usually not tested immediately after collection; rather, the collected samples are stored and transported. Since the virus itself will quickly lyse in vitro and thus affect the results of subsequent testing, virus transport media are required to preserve and transport the virus.
Inactivated virus preservation solution is composed of a virus lysis type preservation solution modified from nucleic acid extraction lysate. It effectively prevents secondary infection by operators and contains inhibitors that protect the viral nucleic acid from degradation, thus enabling subsequent detection by NT-PCR. The inactivated virus transport medium allows viruses to be stored at room temperature for longer, saving the cost of preserving and transporting viral samples.
Non-inactivated virus preservation solution is mainly a modified virus maintenance fluid type preservation solution based on the transport medium. It can maintain the in vitro activity of the virus and the integrity of antigen and nucleic acid and contains Hanks base solution and other antibiotics to protect the virus’s protein shell from decomposition and maintain the originality of virus samples to a greater extent. In addition to nucleic acid extraction and testing, non-inactivated virus transport media can also be used for virus culture, isolation, and antigen detection but requires low-temperature storage if it is to be stored for a long time after sampling.
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The preservation effect, limit of detection (LOD), microbial index, inactivation rate are the key indicators for determining the precision and sens...
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View detailsSingle-use VTM kits are usually divided into two types, non-inactivated and inactivated.
View detailsThe viral transport medium(VTM) has only one mission - to keep the genetic material in the test sample intact.
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