DNA RNA Saliva Collection Kit

Saliva Collection Kit

HIGHLIGHTS

User-friendly, spill-free design allows for safe, convenient collection of saliva/sputum samples. Ideal for at-home self collection applications.

Completely inactivates harmful pathogens at the point of collection, for worry-free transport and downstream analysis.

Stabilizes the DNA and RNA in a sample for transport/storage without the need for cold-chain.

DNA RNA Saliva Collection Kit HIGHLIGHTS

User-friendly, spill-free design allows for safe, convenient collection of saliva/sputum samples. Ideal for at-home self collection applications.

Completely inactivates harmful pathogens at the point of collection, for worry-free transport and downstream analysis.

Stabilizes the DNA and RNA in a sample for transport/storage without the need for cold-chain.

DESCRIPTION

The DNA/RNA Saliva Collection Kit is a user-friendly saliva collection kit for stabilizing the nucleic acid content of saliva and sputum samples. DNA/RNA Shield completely inactivates harmful pathogens in the sample, and stabilizes the RNA in a sample for up to 30 days at room temperature. The design eliminates the risk of spilling the stabilization reagent during use, and is ideal for applications that require at-home self collection.

1. Saliva Collection and Storage

Prior to saliva collection, ensure that the subject’s mouth is free of food or other foreign substances by having the subject rinse their mouth with water and avoiding eating or drinking for 30 min before collecting the sample.

Open a 15 ml centrifuge tube with 2.5 ml of DNA stabilization buffer2 making sure to avoid touching the inside of the cap or tube, and have the subject spit 2.5 ml of saliva into the buffer solution. Note: Collecting more than 2.5 ml of saliva can lead to sample degradation from an insufficient ratio of sample to DNA stabilization buffer. Collecting too little saliva will reduce expected yields from the protocol. To evaluate collection volumes, use the numbered gradients on the side of the tube.

Replace cap and mix by inversion until the mixture is homogenized. Vigorous shaking is not necessary. Store the samples at RT for short-term storage or 4 °C for long-term storage (>3 months).

2. Initial Preparations and Cell Lysis

Prior to starting the extraction, heat a water bath to 37 °C, and prepare an ice bucket. Three 15 ml conical centrifuge tubes will be needed for each extracted sample. The three tubes will be used to hold the cell and protein pellet, the final extracted gDNA, and the isopropanol and ethanol supernatants.

Retrieve samples from storage, and invert samples several times then vortex at medium speed for 15 sec.

Dispense 2.5 ml of sample into a clean 15 ml centrifuge tube, and add 5 ml of Cell Lysis Solution. Mix the sample 50 times by inversion, and incubate at RT for 30 min.