Powdered DMEM Culture Medium

DMEM is developed on the basis of MEM medium. Compared with MEM medium, the content of amino acids has increased by 2 times, and the content of vitamins has increased by 4 times. At the same time, non-essential amino acids, trace iron ions and sodium pyruvate have been added. DMEM medium was initially designed with a glucose content of 1000mg/L (low-sugar type), and later developed a glucose content of 4500mg/L (high-sugar type), which has been widely used in the cultivation of various cells. High-glucose culture medium is widely used in fast growing cells with low adhesion, hybridoma myeloma cells, cloned cells, DNA-transfected transformed cells, host cells of various primary viruses, single cell culture, and vaccine production, such as CHO cell expression of EPO and hepatitis B vaccine production. Low-glucose medium is more suitable for the culture of slower metabolism, dependent adherence cells. Glucose-free medium can adjust the concentration of glucose at will according to research needs, which is convenient and quick.DMEM does not contain proteins, lipids or growth factors. Therefore, DMEM needs to be supplemented with nutrients, usually with 10% fetal bovine serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. For a wide range of cell culture applications, we offer modified DMEM media with various components, as well as DMEM media with customizable components.

Ingredients of Powdered DMEM Culture Medium

This DMEM dry powder cell culture medium needs to be supplemented with sodium bicarbonate, pH adjusted and filtered during preparation. The detailed formulation is as follows:

1. Add 950 mL of distilled water to a mixing vessel as close to final volume as possible.
2. Add dry powdered medium to room temperature (15°C to 30°C) water and stir gently. Do not heat water.
3. Rinse the inside of the pack to remove any residual powder.
4. Add 50mL of 75g/L NaHCO3 solution to the culture medium.
5. Adjust the pH to 0.2 to 0.3 units below the desired final working pH by slowly adding and stirring 1 N NaOH or 1 N HCl. The pH may rise by 0.1 to 0.3 units after filtration. The recommended working pH is 7.0-7.4.
6. Use distilled water to adjust the final volume.
7. Immediately process the culture medium through a 0.2 µm filter membrane into sterile containers using a positive pressure filtration system.