William’s E culture medium was designed by William and Gunn and is mainly used for long-term culture of rat liver epithelial cells, but can also be used for the culture of hepatocytes from other mammals.
L-glutamine (Glutamine) is an essential nutrient element in cell culture, but it is unstable in solution and will degrade spontaneously. The amount of L-glutamine can be arbitrarily adjusted according to the research needs of the medium without L-glutamine, and the addition of fresh L-glutamine or its substitutes at the time of use is more beneficial to the cell growth.
L-alanyl-L-glutamine (Ala-Glu), also known as alanyl-glutamine and alanyl-glutamyl dipeptide, is an advanced cell culture additive that can directly replace L-glutamine in the cell culture medium. l-Glutamine is an essential nutrient in cell culture, but it is unstable in solution and will, however, it is unstable in solution and spontaneously degrades to produce ammonia and pyroglutamic acid, of which ammonia is harmful to cells, whereas L-alanyl-L-glutamine is very stable in aqueous solution and does not degrade spontaneously. The mechanism of cellular utilization is that during cell culture, cells will gradually release a peptidase into the culture medium to hydrolyze L-alanyl-L-glutamyl into L-alanine and L-glutamine, and then cells will take up and utilize these two hydrolysis products. The process of cellular utilization of L-alanyl-L-glutamyl is similar to the flow-addition culture strategy, in that low levels of L-glutamine are continuously added to the culture medium, thereby increasing the utilization of L-glutamine without generating excess ammonia, which is more conducive to cell growth. L-alanyl-L-glutamyl can replace equimolar L-glutamine, is suitable for all cells, requires little adaptation, and can extend the cell culture time. It can also extend the cell culture time and reduce the number of passages, which saves time and money. Cells cultured in medium supplemented with L-glutamine showed a slower reduction in activity than those cultured in a medium supplemented with L-glutamine. The slightly longer delay period is due to the time required for the release of peptidase and digestion of the dipeptide.
Cautions
This product has been filtered and de-bacterized and should be used with care for aseptic operation to avoid contamination. Do not freeze-thaw the product in order to maintain the best use of the product. This product is intended for scientific research or further study use only, not for diagnosis or treatment.
